A multi locus sequence analysis scheme for phylogeny of the Bacillus subtilis species complex and its advantages over 16S rRNA genes

A multi locus sequence analysis (MLSA approach) was studied on the Bacillus genus, or subtilis species complex for specific, including 08 strains from four (B. subtilis, B. pumilus, licheniformis and amyloliquefaciens) were provided by Biotechnology Center of Ho Chi Minh City. The research based sequences 16S rRNA genes, concatenation five protein-coding housekeeping genes: glpF, pta, purH, pycA, rpoD. After PCR amplification sequencing phylogenetic tree sequences, concatenate (as well as each gene) are constructed comparison discussion. aim this study is reach better resolution differentiation within to determine whether MLSA scheme show advantages in gene-based studies.